After cardiac death the potential donor will be assessed by tissue procurement coordinator (a trained medical doctor).
-I.T.B procures tissue aseptically and processes it by strict adherence to surgical principles and clean room protocols. Following tissue recovery, the technologists reconstruct the donor to allow for normal funeral ar¬rangements. Reconstruction will be done by using prosthesis and pads in donor site.
-Tissue retrieval is done within 36 hours of death if the body is refrigerated within 6 hours of death. If the body is kept at room temperature, retrieval will occur within 12 hours of death.
-All allograft tissues are delivered to I.T.B’s clean room facilities in a nutrient medium after recovery. The tissue is processed as soon as possible under clean condition of class 100 laminar flow hood in the class 10/000 clean room. A tissue technician cleans the bone from excess soft tissues and then removes the bone marrow with multiple washing and suctioning. After complete cleaning the tis¬sue is cut into appropriate shapes and each piece packed separately. Multiple samples are taken from bone, attached soft tissues, bone surface swabbing and washing fluid for microbial cultures.
The processed tissue is transferred to ultra low temperature freezer (at –80˚c) for later sterilization.
To minimize cell damage and promote normal function we use cryoprotectant and controlled rate freezing machine with specific protocol for cardiovascular, osteoarticular and soft tissues.
We also use leophylization( freeze-drying)procedure for some musculoskeletal tissues. This procedure can increase tissue’s shelf life.